Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,524,590	G→A	P70L (CCA→CTA)	yicC ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,524,590	0	G	A	100.0%	304.0 / NA	85	P70L (CCA→CTA)	yicC	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/73);  total (12/73)

GCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTGGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAG  >  minE/2524537‑2524630                                                      |                                         gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCTCGCTAACATCTAGCTCATAg                                  <  1:1490334/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:3173029/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:181103/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:1896586/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:1913709/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:1918950/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:19191/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:2288005/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:2609003/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:269951/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:1022944/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:3243974/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:342464/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:3424819/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:3618129/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:514223/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:584539/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:690640/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:784082/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:827522/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:102600/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:1118923/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:142544/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:1324365/62‑1 (MQ=255) gCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTAGCTCATAg                                  <  1:1339034/62‑1 (MQ=255)                                cTCACGTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:34488/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGTGCAGGGTACAt                      <  1:759415/43‑1 (MQ=38)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACaa                      <  1:2775903/43‑2 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2955166/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3121418/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1178676/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3257535/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3338259/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3380241/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1624643/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3422922/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1151980/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2872449/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1151083/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3463732/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3476829/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:363712/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3643824/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:427670/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:463917/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1116218/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:584175/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:60774/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:662792/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:738742/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:776086/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2811882/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1612273/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1721325/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1726256/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1585992/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1874964/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1550023/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:154151/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1494937/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1972014/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:140408/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2382781/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2867730/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2488763/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2549416/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2588122/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:1376550/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2616464/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2421715/43‑1 (MQ=255)                                cTCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2678437/43‑1 (MQ=255)                                 tCACCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:3600011/42‑1 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTAc                        >  1:1107759/1‑38 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:1838700/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:1019477/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:1674615/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:1682929/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:2719484/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:1063288/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:673895/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:3145124/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:1936474/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:2015464/1‑60 (MQ=255)                                   aCCTTGCGCGCTAACATCTAGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAg  >  1:2273269/1‑60 (MQ=255)                                        gcgcgcGAACATCTAGCTCATAGCGCAGGGTACAt                      <  1:2459085/35‑1 (MQ=255)                                                      |                                         GCTGTTTAGCCAGTTTTTCGTTGAGGATCAGCTCACCTTGCGCGCTAACATCTGGCTCATAGCGCAGGGTACATTCCACTTTACCGCGCGTCAG  >  minE/2524537‑2524630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑