Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,935,575	A→T	I72I (ATT→ATA)	idnD ←	L‑idonate 5‑dehydrogenase, NAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,935,575	0	A	T	100.0%	70.5 / NA	23	I72I (ATT→ATA)	idnD	L‑idonate 5‑dehydrogenase, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/23);  total (0/23)

GTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGG  >  minE/2935553‑2935613                       |                                       tgtaTTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:2149872/58‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:3234014/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:998611/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:720102/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:604679/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:538408/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:434335/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:395511/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:377520/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:3604602/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:3391526/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:3282719/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:1143769/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:3229877/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:3199787/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:2937922/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:1803361/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:160311/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:1556310/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:153181/61‑1 (MQ=255) gTAATTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:1374449/61‑1 (MQ=255)   aaTTCTGATGAGTCGCTATGTATAACTTTACCGATAACTTCATGACCTAACACCATCgg  <  1:2819285/59‑1 (MQ=255)   aaTTCTGATGAGTCGCTATGTATAACTTCACCGATAACTTCATGACCTAACACCATCgg  <  1:574441/59‑1 (MQ=255)                       |                                       GTAATTCTGATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGG  >  minE/2935553‑2935613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑