Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,942,498	T→C	Q744R (CAG→CGG)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,942,498	0	T	C	100.0%	261.9 / NA	69	Q744R (CAG→CGG)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/69);  total (0/69)

CTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCC  >  minE/2942448‑2942553                                                   |                                                        cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATTAAGTAGTCCCGGTCGAAGATg                                              <  1:1821197/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3090971/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2656123/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2785899/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2785948/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:282352/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2840288/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2842814/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:108082/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:293706/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2961086/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2965445/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2986720/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3002929/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3064015/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2859809/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3110016/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3480956/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3481509/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3520137/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3584628/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3589707/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:3631004/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:400015/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:501180/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:600329/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:743461/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:835879/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2586958/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:1847781/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:186719/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:1758772/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:1328296/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:1605864/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2096482/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2170043/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2338760/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2354767/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2418167/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2496180/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:2551267/62‑1 (MQ=255) cTGCATAGATGCCTTTGTTGTT‑TCGTCATAAGAGCGGATGAAGTAGTCCCGGTCGAAGATg                                              <  1:55126/61‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTGAGATCTGCCATCTGCGGGcc  <  1:1315115/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:1234981/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:433051/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:3628445/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:542993/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:1301546/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:1268296/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:744964/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:77358/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:801020/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:802976/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:2593847/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:3602839/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:3596311/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:1648153/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:172595/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:3484821/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:1829442/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:3475172/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:3352286/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:3186308/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:3153047/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:1943885/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:199609/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:2061538/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:2095711/62‑1 (MQ=255)                                             tagtCCCGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGcc  <  1:2123018/62‑1 (MQ=255)                                                   |                                                        CTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCC  >  minE/2942448‑2942553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑