Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,006,437	T→C	V107A (GTT→GCT)	ydgH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,006,437	0	T	C	100.0%	89.6 / NA	24	V107A (GTT→GCT)	ydgH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/2);  total (22/2)

AAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCT  >  minE/1006386‑1006462                                                    |                          aaGCCGATTCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGaa                    <  1:3528173/59‑1 (MQ=255) aaGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGaa                    <  1:3452585/59‑1 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCTAAAGATCAGGCt  >  1:2115502/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:3104847/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:808134/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:783921/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:685486/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:458321/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:358706/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:3576987/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:3497861/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:3352996/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:3334048/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:1007002/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:272305/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:2534046/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:2489492/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:2375882/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:2124304/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:2019862/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:1574572/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:149802/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:1247057/1‑62 (MQ=255)                aaGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGCTGTCGAACTGCCGAAAGATCAGGCt  >  1:1146430/1‑62 (MQ=255)                                                    |                          AAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCT  >  minE/1006386‑1006462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑