| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,256,033 | 4 bp→GCCC | coding (1002‑1005/4224 nt) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,256,033 | 0 | C | G | 100.0% | 16.6 / NA | 6 | Q335G (CAG→GGC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (6/0); total (6/0) | |||||||||||
| * | minE | 2,256,034 | 0 | T | C | 100.0% | 17.0 / NA | 6 | Q335G (CAG→GGC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/0); total (6/0) | |||||||||||
| * | minE | 2,256,035 | 0 | G | C | 100.0% | 16.0 / NA | 6 | Q335G (CAG→GGC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (6/0); total (6/0) | |||||||||||
| * | minE | 2,256,036 | 0 | T | C | 100.0% | 17.0 / NA | 6 | K334K (AAA→AAG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/0); total (6/0) | |||||||||||
GTAGTCAACACGCTTACCGAGCAGGTTCTGACGGAAACGACCCTGTTTACCTTTGATCATGT > minE/2255991‑2256052 |||| gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:1077132/1‑60 (MQ=255)gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:1090279/1‑60 (MQ=255)gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:2136741/1‑60 (MQ=255)gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:2905414/1‑60 (MQ=255)gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:3165138/1‑60 (MQ=255)gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:3440336/1‑60 (MQ=255) |||| GTAGTCAACACGCTTACCGAGCAGGTTCTGACGGAAACGACCCTGTTTACCTTTGATCATGT > minE/2255991‑2256052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |