Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,039,486	Δ2 bp	coding (428‑429/654 nt)	ribB ←	3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,039,486	0	C	.	100.0%	228.5 / NA	52	coding (429/654 nt)	ribB	3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (52/0);  total (52/0)
*	minE	2,039,487	0	T	.	100.0%	228.5 / NA	52	coding (428/654 nt)	ribB	3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (52/0);  total (52/0)

CAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGG  >  minE/2039452‑2039515                                   ||                             cAGTATGACCGCCGCGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:101544/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACTACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1029208/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAg                    >  1:1104202/1‑44 (MQ=38) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTg                  >  1:52726/1‑46 (MQ=38) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGaaa             >  1:140729/1‑51 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGcagg   >  1:573030/1‑58 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:778809/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:994884/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:558105/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:564554/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:566683/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:575674/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:648760/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:656382/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:664855/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:708435/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:711112/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:98393/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:812511/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:83101/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:839536/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:844989/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:868836/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:895791/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:909234/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:911071/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1010942/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:971507/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:239223/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1049704/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1069648/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1102169/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1116372/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1127700/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1133137/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1144532/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:1202882/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:168016/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:199947/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:220927/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:522914/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:244101/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:295429/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:30782/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:351121/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:361307/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:401802/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:409025/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:413998/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:450279/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAgg  >  1:509814/1‑62 (MQ=255) cAGTATGACCGCCACGCGTCAGTACACCACCTGC‑‑GAGCGCGAAGTGGGAAAACGTGGCCAg   >  1:253207/1‑61 (MQ=255)                                   ||                             CAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGG  >  minE/2039452‑2039515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑