Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,470,362	+C	coding (1360/3753 nt)	yfaL ←	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,470,358	1	.	C	94.1%	54.3 / ‑2.6	17	coding (1364/3753 nt)	yfaL	adhesin
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (0/16);  total (0/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TATATCGCCGGTTAGCGCCATCGACTGCG‑CCCCTGCCAGCACGTAGCTGCCGTTTTGCGCAA  >  minE/1470330‑1470391                              |                                  tataTCGCCGGTTAGCGCCATCGACTGCG‑CCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:1004396/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:161370/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:90679/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:631423/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:545667/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:524948/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:511515/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:494281/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:474495/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:325209/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:150411/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:138915/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:128105/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:1135465/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:1005767/62‑1 (MQ=255)  atatCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:1005581/62‑1 (MQ=255)     tCGCCGGTTAGCGCCATCGACTGCGCCCCCTGCCAGCACGTAGCTGCCGTTTTGCGCaa  <  1:134999/59‑1 (MQ=255)                              |                                  TATATCGCCGGTTAGCGCCATCGACTGCG‑CCCCTGCCAGCACGTAGCTGCCGTTTTGCGCAA  >  minE/1470330‑1470391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑