Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,381,384	+A	intergenic (‑104/+37)	rmuC ← / ← udp	predicted recombination limiting protein/uridine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,381,380	1	.	A	100.0%	42.6 / NA	14	intergenic (‑100/+41)	rmuC/udp	predicted recombination limiting protein/uridine phosphorylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/14);  total (0/14)

AGGCGCTACGCAAAAATAC‑AAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAT  >  minE/2381362‑2381421                    |                                          aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCATACAGGAGAAGAGAAt  <  1:1062104/61‑1 (MQ=38) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:1054785/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:1056878/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:1058808/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:1190160/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:183981/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:19109/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:206061/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:420127/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:8038/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:827543/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:920935/61‑1 (MQ=255) aGGCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:97535/61‑1 (MQ=255)  ggCGCTACGCAAAAATACAAAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAt  <  1:848677/60‑1 (MQ=38)                    |                                          AGGCGCTACGCAAAAATAC‑AAAAGGCCGAACGCGTCGGCCTTCAGACAGGAGAAGAGAAT  >  minE/2381362‑2381421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑