Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,440,936	+T	coding (144/894 nt)	ilvY →	DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,440,933	1	.	T	100.0%	130.3 / NA	39	L47L (CTG→CTT)	ilvY	DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (39/0);  total (39/0)

AAGAAGATCTCGGTCAGCCGCTG‑TTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGC  >  minE/2440911‑2440971                        |                                       aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAAc                         >  1:1189780/1‑39 (MQ=37) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:757324/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:506012/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:544942/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:545510/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:584285/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:59046/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:665015/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:695617/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:698758/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:698876/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:492879/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:76387/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:77706/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:86465/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:887901/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:920336/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:927167/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:94296/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:943868/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:255629/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:1030835/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:1157540/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:1172567/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:207129/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:214392/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:218320/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:249256/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:252912/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:502424/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:303065/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:318596/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:319020/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:326839/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:450315/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:469827/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:481447/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc  >  1:1026322/1‑62 (MQ=255) aagaagATCTCGGTCAGCCGCTGTTTTGTGCACGATAACCGCACGGTGACGCTGACTGAAGc  >  1:1147706/1‑62 (MQ=255)                        |                                       AAGAAGATCTCGGTCAGCCGCTG‑TTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGC  >  minE/2440911‑2440971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑