| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,469,157 | (AGC)3→4 | coding (139/1383 nt) | atpD → | F1 sector of membrane‑bound ATP synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,469,147 | 1 | . | C | 85.7% | 63.1 / 4.6 | 21 | coding (129/1383 nt) | atpD | F1 sector of membrane‑bound ATP synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (0/18); total (0/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | minE | 2,469,147 | 2 | . | A | 85.7% | 53.6 / 5.6 | 21 | coding (129/1383 nt) | atpD | F1 sector of membrane‑bound ATP synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base A (0/18); total (0/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
| * | minE | 2,469,147 | 3 | . | G | 85.7% | 60.8 / 5.0 | 21 | coding (129/1383 nt) | atpD | F1 sector of membrane‑bound ATP synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base G (0/18); total (0/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
AAATGGTAATGAGCGTCTGGTGCTGGAAGTT‑‑‑CAGCAGCAGCTCGGCGGCGGTATCGTACGTA > minE/2469117‑2469178 ||| aaaTGGTAATGAGCGTCTGGTGCTGGAAGTT‑‑‑CAGCAGCAGCTCGGCGGCGGTATcgtacgta < 1:1203192/62‑1 (MQ=255)aaaTGGTAATGAGCGTCTGGTGCTGGAAGTT‑‑‑CAGCAGCAGCTCGGCGGCGGTATcgtacgta < 1:1409/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:456006/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:927133/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:788163/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:684990/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:63509/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:617053/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:604239/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:5336/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:496443/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:487727/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:1003510/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:264469/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:258709/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:206739/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:19810/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:1169906/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:1077677/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTTCAGCAGCAGCAGCTCGGCGGCGGTATcgtacg < 1:1013035/62‑1 (MQ=255) aaTGGTAATGAGCGTCTGGTGCTGGAAGTT‑‑‑CAGCAGCAGCTCGGCGGCGGTATcgtacgta < 1:689128/61‑1 (MQ=255) ||| AAATGGTAATGAGCGTCTGGTGCTGGAAGTT‑‑‑CAGCAGCAGCTCGGCGGCGGTATCGTACGTA > minE/2469117‑2469178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |