Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,307,0200TC58.7% 51.4 / 108.3 92P192P (CCA→CCGyeeFpredicted amino‑acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/38);  new base C (54/0);  total (54/38)
Fisher's exact test for biased strand distribution p-value = 9.71e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAA  >  minE/1306962‑1307075
                                                          |                                                       
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCTGACCAGAACgg                                                       >  1:65224/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:469017/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:72546/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:68676/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:612320/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:607465/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:572432/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:54292/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:533477/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:736241/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:465595/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:460751/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:453724/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:445131/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:43439/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:407265/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:335136/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:334757/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:854350/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:982904/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:978332/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:964442/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:958389/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:94482/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:938144/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:873014/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:862100/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:1013017/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:830503/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:827941/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:797716/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:791575/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:790658/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:773756/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:739514/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:250312/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:1145709/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:186955/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:187928/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:19087/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:1150041/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:110609/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:179582/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:332886/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:228492/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:24309/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:251634/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:12337/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:320779/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:1067743/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:134182/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:137966/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:1026809/1‑61 (MQ=255)
aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAACgg                                                       >  1:1010540/1‑61 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCTTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:514542/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCacaaacaa  <  1:269343/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:1152432/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:751533/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:820247/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:723618/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:84420/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:1125990/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:869205/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:1096446/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:878375/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:926435/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:1073861/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:1065425/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:961025/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:1040266/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:199018/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:326957/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:361470/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:374091/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:39634/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:402801/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:323369/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:279663/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:464720/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:507369/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:220839/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:742206/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:57932/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:605341/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:615078/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:6519/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:686431/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:725095/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:154602/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:145599/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:738923/62‑1 (MQ=255)
                                                    ccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAaacaa  <  1:14123/62‑1 (MQ=255)
                                                          |                                                       
AGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAA  >  minE/1306962‑1307075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: