| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,601,390 | 1 | . | A | 35.7% | 28.7 / 40.8 | 28 | coding (231/1305 nt) | yfeW | predicted periplasmic esterase |
| Reads supporting (aligned to +/- strand): ref base . (0/18); new base A (0/10); total (0/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGC > minE/1601346‑1601424 | aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:100974/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:628924/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:549523/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:46543/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:428851/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:381103/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:372946/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:33977/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:294937/62‑1 (MQ=255)aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAAGTACGATGGCa < 1:1004807/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:288151/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:972442/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:864296/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:813694/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:770242/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:760382/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:704173/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:549169/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:547824/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:1033689/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:1033936/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:407954/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:265863/62‑1 (MQ=255) tgtgTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:353844/62‑1 (MQ=255) gtgtATCGCAAAGCCTGGTGAGCGGCG‑AAATAGTACGATGGCAGCGTGTTGATGGAACAgg < 1:784424/61‑2 (MQ=255) gtgtATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:1018436/61‑1 (MQ=255) gtgtATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:569074/61‑1 (MQ=255) gtgtATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGc < 1:1150454/61‑1 (MQ=255) | ATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCG‑AAAAAGTACGATGGCAGCGTGTTGATGGAACAGC > minE/1601346‑1601424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |