Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,657,2920GA73.6% 264.1 / 111.3 159E521E (GAG→GAA) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base G (6/36);  new base A (117/0);  total (123/36)
Fisher's exact test for biased strand distribution p-value = 8.04e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 159 total aligned reads displayed.

CGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACC  >  minE/1657260‑1657331
                                |                                       
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGcc                                   >  1:1150376/1‑39 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCa                                  >  1:48862/1‑40 (MQ=255)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGTGCAGCAAGGGCTGccc              >  1:370190/1‑60 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:871352/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:507212/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:492719/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:4856/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:445685/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:434567/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:866769/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:418211/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:511722/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:408510/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:385725/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:37995/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:361234/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:880884/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:88207/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:89142/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:293965/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:598810/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:739843/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:729962/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:71355/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:762053/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:692147/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:781057/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:634876/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:783277/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1000247/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:79476/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:576033/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:570468/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:563017/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:561126/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:5477/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:841560/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:514169/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:899183/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:100822/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1017702/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1038286/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1058477/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1106067/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1120250/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1122269/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:974225/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:971475/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1142796/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:962331/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1203070/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:121163/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:949951/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:130087/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:143869/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:284140/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:257196/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:251250/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:24096/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:237276/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:23151/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:903816/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:139585/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:917083/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:185010/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:181597/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGcc               >  1:1008581/1‑59 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGCGCTGccc              >  1:986284/1‑60 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCAAACGCgcagc                         >  1:1067956/1‑49 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACAGCGAAATCGCCAACGCGAAGCAAGGGCTGccc              >  1:554929/1‑60 (MQ=255)
cgccgcCTGTTGTATGAAATGGAGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc              >  1:1094315/1‑60 (MQ=38)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCTCAGCAAGGGCTGCCCAGTgg         <  1:349806/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCTGCAAGGGCTGCCCAGTgg         <  1:234532/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:976451/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:91475/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:824875/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:1001024/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:756805/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:312871/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:1024915/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:1081853/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:11570/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:127376/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:15697/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:227873/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:272763/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:749042/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:321299/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:348905/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:408858/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:533448/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:709706/52‑1 (MQ=255)
             aTGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTACCCAGTgg         <  1:581110/52‑1 (MQ=255)
              tGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:420861/51‑1 (MQ=255)
              tGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:217491/51‑1 (MQ=255)
              tGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg         <  1:641686/51‑1 (MQ=255)
                    ggtggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAcc  >  1:599901/1‑52 (MQ=255)
                    ggtggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAcc  >  1:1140654/1‑52 (MQ=255)
                    ggtggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAcc  >  1:113272/1‑52 (MQ=255)
                    ggtggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAc   >  1:521914/1‑51 (MQ=255)
                                |                                       
CGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACC  >  minE/1657260‑1657331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑