Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,763,5800GA69.8% 6.0 / 100.3 119noncoding (264/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (36/0);  new base A (0/83);  total (36/83)
Fisher's exact test for biased strand distribution p-value = 2.63e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

GGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCA  >  minE/1763532‑1763605
                                                |                         
ggCTGGTCATCCTCTCAGACCAGCCAGTGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:117889/1‑60 (MQ=11)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGtt                         >  1:758364/1‑51 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:39053/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:782165/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:244535/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:72317/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:710637/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:811432/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:146519/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:306776/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:365718/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:37396/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:644594/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:1199310/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:633572/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:886208/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:890678/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:907843/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:917707/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:924352/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:588132/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:1096592/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:951849/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:1083327/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:1039172/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:435512/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:482895/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:500646/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:534995/1‑60 (MQ=255)
ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTT‑GTGAGCCGTTACCCCACcaaca             >  1:743105/1‑62 (MQ=14)
   gggTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaacaa            >  1:204332/2‑61 (MQ=255)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:646543/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:977353/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:970540/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:964980/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:568658/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:953139/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:58810/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:934381/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:592715/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:865806/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:865418/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:862207/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:828837/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:427082/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:817549/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:65265/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:655685/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:65594/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:813975/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:708110/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:710448/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:808535/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:720721/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:808448/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:806286/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:72608/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1184170/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:513782/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:199495/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:171700/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:167011/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:152681/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:139398/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:127255/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:126877/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:125909/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:213640/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1174389/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1165344/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1155621/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1148843/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1124781/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1032448/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1031519/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1027279/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1021955/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:338330/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:501487/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:47057/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:430869/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1019923/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:39537/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:385239/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:384510/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:369938/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:218049/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:335603/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:319027/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:299234/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:285142/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:264185/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:260011/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:233724/61‑3 (MQ=11)
             ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:219469/61‑3 (MQ=11)
              tcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCATCTAGCTATTccga  <  1:250073/60‑3 (MQ=2)
              tcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1117311/60‑3 (MQ=11)
                aGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1148326/58‑3 (MQ=11)
                           ggATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:660922/47‑3 (MQ=11)
                           ggATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1093969/47‑3 (MQ=11)
                                                |                         
GGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCA  >  minE/1763532‑1763605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑