| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,856,878 | 0 | T | . | 62.5% | 25.3 / 51.0 | 32 | intergenic (‑177/+182) | ygcB/cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base T (12/0); new base . (0/20); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCC > minE/1856830‑1856919 | gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCttt > 1:64861/1‑57 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGt > 1:528179/1‑60 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:904499/1‑62 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:610588/1‑62 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:739746/1‑62 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:78934/1‑62 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:852934/1‑62 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:173406/1‑62 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:889250/1‑62 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:146612/1‑62 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc > 1:560321/1‑61 (MQ=255)gAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc > 1:1170720/1‑61 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:871258/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:883669/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:752794/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:728505/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:727056/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:71306/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:659533/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:580591/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:59354/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:1021218/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:505340/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:46503/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:452054/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:406386/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:388924/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:342770/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:227341/62‑1 (MQ=255) cAGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:169259/62‑1 (MQ=255) aGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:558650/61‑1 (MQ=255) aGGTTGATAGAAATATCGCC‑GGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGcc < 1:105084/61‑1 (MQ=255) | GAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCC > minE/1856830‑1856919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |