Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,043,4191.A47.7% 7.0 / 183.9 88coding (499/630 nt)yqiJpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/46);  new base A (0/42);  total (0/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 106 total aligned reads displayed.

ACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAG  >  minE/2043359‑2043470
                                                             |                                                   
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGcc                                                      >  1:466268/1‑61 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGcc                                                      >  1:1124251/1‑61 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:931779/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:612590/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:676242/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:507627/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:685411/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:701625/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:723048/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:264271/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:216742/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:769116/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:167079/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:139906/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:1069476/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:823640/1‑62 (MQ=255)
aCCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCa                                                    >  1:869925/1‑62 (MQ=255)
       atcaAGCCACTTCAGGTATTCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:693070/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:433709/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:362957/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:365181/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:370824/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:388789/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:870025/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:431639/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:431658/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:683461/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:433021/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:687581/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:454365/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:884858/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:472362/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:895078/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:666181/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:635297/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:56322/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:585473/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:21792/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:1188106/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:786108/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:183713/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:185524/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:212972/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:767742/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:851686/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:361555/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:235379/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:730850/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:245932/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:114510/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:858283/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:308467/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:318153/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:344231/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:797598/62‑1 (MQ=255)
       atcaAGCCACTTCAGGTAATCCCTGCGAACGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:785742/62‑1 (MQ=255)
        tcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:960333/61‑1 (MQ=255)
        tcaAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:359689/61‑1 (MQ=255)
         caAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:136465/60‑1 (MQ=255)
                          tCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACat                                             <  1:67414/43‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:88328/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:796340/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:799217/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:1157837/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:1156590/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:1135153/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:1123571/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:110695/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:878405/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:879047/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:58929/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:1084688/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:888878/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:1078451/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:89933/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:915161/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:92499/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:1011297/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:1004535/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:713915/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:579429/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:577/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:632214/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:551562/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:524959/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:432582/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:420398/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:308347/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:167225/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:304886/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:733591/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:753258/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:213826/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:191267/62‑1 (MQ=255)
                                                   cAATTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:187148/62‑1 (MQ=255)
                                                    aaTTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:242314/61‑1 (MQ=255)
                                                    aaTTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:309681/61‑1 (MQ=255)
                                                    aaTTTGGCCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAg  <  1:518159/61‑1 (MQ=255)
                                                      tttGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTc         <  1:797502/51‑1 (MQ=255)
                                                      tttGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTc         <  1:785913/51‑1 (MQ=255)
                                                             |                                                   
ACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAG  >  minE/2043359‑2043470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: