| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,349,399 | 0 | G | C | 30.0% | 25.2 / 13.4 | 20 | T750S (ACC→AGC) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base G (14/0); new base C (0/6); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGTTG > minE/2349342‑2349441 | cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:1116756/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:794408/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:692790/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:661213/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:647025/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:1118117/62‑1 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:191080/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:490371/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:550007/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:632887/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:1170215/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:670252/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:708470/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:748711/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:1026709/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:835952/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:958437/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:963221/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGtt > 1:99913/1‑62 (MQ=255) cgcTACGGCGTTGCTCGCT‑GTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGTTg > 1:1123711/1‑62 (MQ=255) | CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGACGGTTTCCAGTGGCAAACCAAACACTTCTGCCGCCGTTG > minE/2349342‑2349441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |