Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	834,583	C→T	S1014F (TCT→TTT)	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	834,583	0	C	T	100.0%	57.9 / NA	19	S1014F (TCT→TTT)	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/19);  total (0/19)

GCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAA  >  minE/834529‑834613                                                       |                               gCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTggtttggtct                          >  1:105126/1‑61 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:911306/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:776286/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:774885/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:655383/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:533724/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:504118/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:408254/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:369558/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:3630/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:285164/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:28340/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:2536/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:163234/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:1123627/62‑1 (MQ=255)                        ccGCGCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:1065999/62‑1 (MQ=255)                         cgcgCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:1171506/61‑1 (MQ=255)                         cgcgCAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:733607/61‑1 (MQ=255)                             cAAAATTATCTCCAGCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:280972/57‑1 (MQ=255)                                           gCCCGACCTGGTTTGGTCTGGAAGATGAACACGTTTCTTACaa  <  1:1024712/43‑1 (MQ=255)                                                       |                               GCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAA  >  minE/834529‑834613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑