Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,319,661	G→A	V77V (GTC→GTT)	ugd ←	UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,319,661	0	G	A	100.0%	74.2 / NA	23	V77V (GTC→GTT)	ugd	UDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/23);  total (0/23)

GCGATGATGACATAATCAGCATCCCGGTAGGCTTCATTTTTAT  >  minE/1319653‑1319695         |                                   gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:452560/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:992915/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:940007/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:889192/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:850815/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:730381/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:709917/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:668763/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:604289/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:580693/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:553026/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:486408/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:106237/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:44312/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:42382/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:26275/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:232031/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:224651/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:158203/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:134429/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:1201436/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:1167415/43‑1 (MQ=255) gCGATGATAACATAATCAGCATCCCGGTAGGCTTCATTTTTAt  <  1:1113487/43‑1 (MQ=255)         |                                   GCGATGATGACATAATCAGCATCCCGGTAGGCTTCATTTTTAT  >  minE/1319653‑1319695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑