Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,456,354	A→T	D332E (GAT→GAA)	yfaQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,456,354	0	A	T	100.0%	161.9 / NA	51	D332E (GAT→GAA)	yfaQ	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/38);  total (13/38)

GGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTC  >  minE/1456309‑1456405                                              |                                                    ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGCACTCTGGCac                                     >  1:792220/1‑61 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:1035504/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:1035016/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:600398/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:549462/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:499465/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:165317/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:944407/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:374541/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCaa                                     >  1:363229/1‑62 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCa                                      >  1:1193918/1‑61 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCa                                      >  1:924166/1‑61 (MQ=255) ggTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGTTCCAGAACTCTGGCa                                      >  1:360284/1‑61 (MQ=255)                                     cgccgcGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:291670/61‑1 (MQ=255)                                     cgccgcGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:739562/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:99248/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:826097/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:818014/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:840200/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:85986/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:92096/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:77981/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:928156/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:952551/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:972602/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:765769/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:75503/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:977739/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:989328/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:696856/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:655007/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:1129868/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:1189311/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:1189437/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:152502/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:157357/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:200656/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:236279/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:238898/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:25070/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:261644/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:267038/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:308066/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:319138/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:351315/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:373422/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:592973/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:598188/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:605439/61‑1 (MQ=255)                                     cGCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:676521/61‑1 (MQ=255)                                      gCCTCGCGTTCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTc  <  1:843932/60‑1 (MQ=255)                                              |                                                    GGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTC  >  minE/1456309‑1456405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑