Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,657,268 A→G L513L (CTA→CTG)  ppk → polyphosphate kinase, component of RNA degradosome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,657,2680AG100.0% 427.7 / NA 117L513L (CTA→CTG) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (117/0);  total (117/0)

Only 100 of 117 total aligned reads displayed.

CGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCC  >  minE/1657260‑1657319
        |                                                   
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGcc                       >  1:1150376/1‑39 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCaa                     >  1:1072571/1‑41 (MQ=255)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCa                      >  1:48862/1‑40 (MQ=255)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCa                      >  1:1039736/1‑40 (MQ=255)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGTGCAGCAAGGGCTGccc  >  1:370190/1‑60 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:531396/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:71355/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:706631/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:692147/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:6646/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:634876/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:598810/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:5962/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:576033/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:570468/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:563017/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:550842/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:5477/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:746279/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:514169/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:511722/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:507212/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:498433/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:492719/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:4856/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:445685/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:434567/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:873131/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:974225/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:971475/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:962331/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:959580/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:917083/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:903816/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:899183/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:892096/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:89142/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:886549/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:880884/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:729962/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:871352/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:866769/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:844423/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:841560/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:789425/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:783277/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:781057/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:762053/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:749418/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:100219/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1128551/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:181597/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:171398/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:143869/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:142052/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:139585/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:130087/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:121163/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1203070/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:118203/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1144245/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1141823/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:420622/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1122269/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1120250/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1114239/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1106067/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1058477/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1038286/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1017702/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1015019/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:100822/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1000247/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:226968/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:421949/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:418211/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:408510/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:385725/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:384455/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:37995/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:377173/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:35117/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:293965/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:284140/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:263463/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:257196/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:24096/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:23885/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:237276/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:232411/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:23151/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:185010/1‑60 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGcc   >  1:1008581/1‑59 (MQ=39)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGcac  >  1:579516/1‑58 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGCGCTGccc  >  1:986284/1‑60 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACCGCGAAATCGCAAACGCgcagc             >  1:1067956/1‑49 (MQ=38)
cgccgcCTGTTGTATGAAATGGTGGACAGCGAAATCGCCAACGCGAAGCAAGGGCTGccc  >  1:554929/1‑60 (MQ=255)
cgccgcCTGTTGTATGAAATGGAGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:1094315/1‑60 (MQ=38)
cgccgcCTGTTGTATGAAATGCTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:555570/1‑60 (MQ=38)
cgccgcCTGTGGTATGAAATGGTGGACCGCGAAATCGCCAACGCGCAGCAAGGGCTGccc  >  1:348639/1‑60 (MQ=38)
        |                                                   
CGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCC  >  minE/1657260‑1657319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑