Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,763,558 G→C noncoding (286/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,763,5580GC81.6% 136.3 / 97.3 196noncoding (286/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (36/0);  new base C (79/81);  total (115/81)
Fisher's exact test for biased strand distribution p-value = 3.95e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

Only 100 of 196 total aligned reads displayed.

TTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCA  >  minE/1763523‑1763605
                                   |                                               
ttCCAGTGTGACTGATCATCCTCTCAGCCCAGTTACGGATCGTCGCCTTGGTg                                >  1:67680/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:429511/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:627348/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:579069/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:567735/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:525909/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:491375/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:481279/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:468031/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:651700/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1005495/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:412344/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:386700/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:355892/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:344772/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:319869/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:317609/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:428586/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:710325/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:733371/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:738835/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:747803/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:77844/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:788986/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:822607/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:833936/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:850509/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:864738/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:92420/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:930943/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:977326/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:990908/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1074949/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1147028/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1139884/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1111432/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1088516/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:118642/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1197782/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1199577/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:128960/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:16192/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1005636/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:225753/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTg                                >  1:1035857/1‑53 (MQ=11)
ttCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTAGCCTTGGTg                                >  1:503500/1‑53 (MQ=11)
         ggCTGGTCATCCTCTCAGACCAGCCAGTGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:117889/1‑60 (MQ=11)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:365718/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:710637/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:244535/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:633572/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:588132/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:534995/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:500646/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:907843/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:37396/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:435512/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:890678/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:886208/1‑60 (MQ=255)
         ggCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                >  1:1039172/1‑60 (MQ=255)
            gggTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaacaa            >  1:204332/2‑61 (MQ=255)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:953139/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:865806/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1060346/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:941381/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:964980/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:646543/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:813975/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:808535/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:806286/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1021955/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1124781/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:828837/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:983848/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1031519/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:72608/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:264185/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:285142/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:299234/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:219469/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:338330/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:213640/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:345890/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:369938/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:385239/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:194948/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:39537/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:167011/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:152681/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:139398/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:126877/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:634116/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1174389/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:655685/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:658724/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1165344/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1155621/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:720777/61‑3 (MQ=11)
                      ctcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATccga  <  1:1148843/61‑3 (MQ=11)
                       tcAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACCTCACCATCTAGCTATTccga  <  1:250073/60‑3 (MQ=2)
                                   |                                               
TTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCA  >  minE/1763523‑1763605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑