Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,896,276	G→A	intergenic (‑229/‑3)	amiC ← / → argA	N‑acetylmuramoyl‑L‑alanine amidase/fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,896,276	0	G	A	100.0%	187.9 / NA	56	intergenic (‑229/‑3)	amiC/argA	N‑acetylmuramoyl‑L‑alanine amidase/fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/56);  total (0/56)

TACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCGAGT  >  minE/1896242‑1896303                                   |                            tACAATAATTTCGAATAATCATGCAAAGATGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1078477/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:586692/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:331528/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1006332/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:399613/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:406475/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:465735/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:473747/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:52429/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:549749/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:562745/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:575523/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:577094/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:579935/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:381982/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:626395/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:650475/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:658580/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:7048/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:790029/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:815211/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:857315/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:869606/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:913108/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:960047/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:963473/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1147315/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1007526/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1007687/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1015492/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1032565/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1045555/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1083999/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1088785/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:109714/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1098336/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:111554/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:114568/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1183494/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:124093/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:326059/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:282670/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:271523/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:265900/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:177370/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:175189/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGGGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:300387/62‑2 (MQ=255) tACAATAATTTCGAATAATCATGCAAAGAGGAGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:511596/62‑2 (MQ=255)  acaaTAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:24110/61‑2 (MQ=255)  acaaTAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:754588/61‑2 (MQ=255)  acaaTAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:266665/61‑2 (MQ=255)  acaaTAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:452470/61‑2 (MQ=255)  acaaTAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:1184795/61‑2 (MQ=255)      tAATTTCGAATAATCATGGAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:326234/57‑2 (MQ=255)           tCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:740180/52‑2 (MQ=255)                           agagGTGTACCGTGGTAAAGGAACGTAAAACCGAgc  <  1:736956/36‑2 (MQ=39)                                   |                            TACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCGAGT  >  minE/1896242‑1896303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑