Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,168,887	G→T	D139Y (GAT→TAT)	yhbN →	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,168,887	0	G	T	100.0%	145.6 / NA	44	D139Y (GAT→TAT)	yhbN	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/36);  total (8/36)

TAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCA  >  minE/2168880‑2168944        |                                                          tAAGGGCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACaaa      >  1:980171/1‑61 (MQ=255) tAAGGGCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACaaa      >  1:980072/1‑61 (MQ=255) tAAGGGCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACaaa      >  1:1060988/1‑61 (MQ=255) tAAGGGCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACaaa      >  1:1013341/1‑61 (MQ=255) tAAGGGCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACaaa      >  1:1094674/1‑61 (MQ=255) tAAGGGCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACaaa      >  1:619830/1‑61 (MQ=255) tAAGGGCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACaaa      >  1:679387/1‑61 (MQ=255) tAAGGGCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACaaa      >  1:265607/1‑61 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:763814/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:551673/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:567194/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:640755/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:704834/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:732367/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:743629/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:751291/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:508570/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:777839/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:793421/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:904136/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:904160/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:912348/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:91970/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:980827/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:190310/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1035849/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1043254/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1053406/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1087626/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1102660/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1118136/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1123846/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1163562/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:1178162/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:538135/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:22812/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:228225/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:335337/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:344651/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:360374/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:411933/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:492333/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCa  <  1:523407/61‑1 (MQ=255)     ggCTATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGAAGGCTTTCAGCGACAAAGGCa  <  1:267161/61‑1 (MQ=255)        |                                                          TAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCA  >  minE/2168880‑2168944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑