Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,304,347	A→G	K113E (AAA→GAA)	yiiX →	predicted peptidoglycan peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,304,347	0	A	G	100.0%	141.9 / NA	40	K113E (AAA→GAA)	yiiX	predicted peptidoglycan peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/40);  total (0/40)

AGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTAAACCGTATGATTTCAGCTTCTCA  >  minE/2304309‑2304370                                       |                        aGCAAATACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1118254/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:660054/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:94825/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:398168/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:427483/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:432452/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:545031/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:572355/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:616396/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:636819/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:374597/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:676269/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:734981/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:829342/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:862003/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:865328/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:919893/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:938313/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:197126/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1013324/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1079844/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1098922/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1125199/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1130449/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1144760/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1185100/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:142351/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:34150/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:200874/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:227138/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:228242/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:233381/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:260120/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:285222/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:285421/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGACAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:556449/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGACAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:727421/62‑1 (MQ=255) aGCAAAAACTGGCGCAAACGACAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:894752/62‑1 (MQ=255)  gCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:1062373/61‑1 (MQ=255)        aCTGGCGCAAACGGCAAAACGTTATCTCGGTGAACCGTATGATTTCAGCTTCTCa  <  1:393907/55‑1 (MQ=255)                                       |                        AGCAAAAACTGGCGCAAACGGCAAAACGTTATCTCGGTAAACCGTATGATTTCAGCTTCTCA  >  minE/2304309‑2304370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑