| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,349,374 | T→C | K758K (AAA→AAG) | polA ← | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,349,374 | 0 | T | C | 100.0% | 16.3 / NA | 6 | K758K (AAA→AAG) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/6); total (0/6) | |||||||||||
CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGAC > minE/2349342‑2349403 | cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:1116756/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:1118117/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:647025/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:661213/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:692790/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:794408/62‑1 (MQ=255) | CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGAC > minE/2349342‑2349403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |