Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | minE | 2,349,377 | C→G | A757A (GCG→GCC) | polA ← | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,349,377 | 0 | C | G | 100.0% | 15.6 / NA | 6 | A757A (GCG→GCC) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (0/6); total (0/6) |
CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGAC > minE/2349342‑2349403 | cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:1116756/62‑1 (MQ=255) cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:1118117/62‑1 (MQ=255) cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:647025/62‑1 (MQ=255) cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:661213/62‑1 (MQ=255) cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:692790/62‑1 (MQ=255) cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:794408/62‑1 (MQ=255) | CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGAC > minE/2349342‑2349403 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |