Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,349,377 C→G A757A (GCG→GCC polA ← fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,349,3770CG100.0% 15.6 / NA 6A757A (GCG→GCCpolAfused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/6);  total (0/6)

CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGAC  >  minE/2349342‑2349403
                                   |                          
cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc  <  1:1116756/62‑1 (MQ=255)
cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc  <  1:1118117/62‑1 (MQ=255)
cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc  <  1:647025/62‑1 (MQ=255)
cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc  <  1:661213/62‑1 (MQ=255)
cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc  <  1:692790/62‑1 (MQ=255)
cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc  <  1:794408/62‑1 (MQ=255)
                                   |                          
CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGAC  >  minE/2349342‑2349403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: