| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,353,663 | G→T | A251E (GCA→GAA) | yihF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,353,663 | 0 | G | T | 95.4% | 197.0 / 2.4 | 65 | A251E (GCA→GAA) | yihF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base T (62/0); total (65/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGTAGGTGAGATTGACATTGATATAATCTTTGTCTTTAACGCGATCCAGCCTGGTTTTTGCAACGAAAGCATCGATTTGGGCAACGTCTTCCCC > minE/2353604‑2353697 | cGTAGGTGAGATTGACATTGATATAATCTTTGTCTTTAACGCGATCCAGCCTGGTTTTTGCa > 1:479672/1‑62 (MQ=255)cGTAGGTGAGATTGACATTGATATAATCTTTGTCTTTAACGCGATCCAGCCTGGTTTTTGCa > 1:419499/1‑62 (MQ=255)cGTAGGTGAGATTGACATTGATATAATCTTTGTCTTTAACGCGATCCAGCCTGGTTTTTGCa > 1:514054/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGc > 1:41468/1‑38 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATc > 1:329596/1‑41 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:729529/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:730583/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:717386/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:699519/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:681654/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:675852/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:579942/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:572050/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:545965/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:520964/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:510979/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:48273/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:452628/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:739661/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:759722/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:762570/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:773932/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:784452/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:814481/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:820795/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:874640/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:891790/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:895530/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:899282/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:915608/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:920820/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:943907/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:994928/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1148267/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:102725/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1032156/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1051238/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1056313/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:110264/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1102687/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1102866/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1111319/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1112605/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1113212/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1115721/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1119665/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1130733/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:452652/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:239398/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1023815/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:371830/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:34478/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:310909/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:284157/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:267403/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:247421/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1169529/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:219785/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:216984/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:205839/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:125488/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1186910/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTcccc > 1:1183915/1‑62 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTccc > 1:780073/1‑61 (MQ=255) tCTTTAACGCGATCCAGCCTGGTTTTTTCAACGAAAGCATCGATTTGGGCAACGTCTTaccc > 1:937316/1‑62 (MQ=255) | CGTAGGTGAGATTGACATTGATATAATCTTTGTCTTTAACGCGATCCAGCCTGGTTTTTGCAACGAAAGCATCGATTTGGGCAACGTCTTCCCC > minE/2353604‑2353697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |