| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,445,047 | G→C | A30G (GCC→GGC) | ilvD ← | dihydroxyacid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,445,047 | 0 | G | C | 100.0% | 16.0 / NA | 6 | A30G (GCC→GGC) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (0/6); total (0/6) | |||||||||||
GATAATCGGCTTACCGAAATCGGCGTCGGTCATTCCGGTGGCGCGCCACAGCGCACGAGCAC > minE/2445025‑2445086 | gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:236511/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:447497/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:459721/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:466005/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:630416/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:925371/62‑3 (MQ=255) | GATAATCGGCTTACCGAAATCGGCGTCGGTCATTCCGGTGGCGCGCCACAGCGCACGAGCAC > minE/2445025‑2445086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |