Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,468,203	G→A	E25E (GAG→GAA)	atpG →	F1 sector of membrane‑bound ATP synthase, gamma subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,468,203	0	G	A	100.0%	49.1 / NA	16	E25E (GAG→GAA)	atpG	F1 sector of membrane‑bound ATP synthase, gamma subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/0);  total (16/0)

AAGATCACTAAAGCGATGGAGATGGTCGCCGCTTCCAAAATGCGTAAATCGCAGGATCGCAT  >  minE/2468183‑2468244                     |                                          aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAAt                       >  1:403068/1‑41 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCa   >  1:722772/1‑61 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCa   >  1:974987/1‑61 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:1059549/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:1154262/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:12587/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:17261/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:222918/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:364516/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:415620/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:470438/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:471405/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:701830/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:741164/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:861180/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt  >  1:996161/1‑62 (MQ=255)                     |                                          AAGATCACTAAAGCGATGGAGATGGTCGCCGCTTCCAAAATGCGTAAATCGCAGGATCGCAT  >  minE/2468183‑2468244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑