| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,925,224 | T→A | K147N (AAA→AAT) | holC ← | DNA polymerase III, chi subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,925,224 | 0 | T | A | 96.5% | 88.8 / ‑3.4 | 29 | K147N (AAA→AAT) | holC | DNA polymerase III, chi subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (28/0); total (28/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
AGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCA > minE/2925181‑2925243 | aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:552267/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:997615/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:989132/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:910363/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:90696/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:899779/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:898190/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:839415/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:785506/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:672620/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:659568/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:653122/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:621096/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:61611/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:592268/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:1015870/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:467787/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:403980/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:343537/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:232052/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:173803/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:143196/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:114734/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:1084580/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:1079722/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:107423/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:106235/1‑62 (MQ=255)aGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTAATTCCAGGTTGCCGTATTc > 1:102232/1‑62 (MQ=255) gCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCa < 1:401581/62‑1 (MQ=255) | AGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCA > minE/2925181‑2925243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |