Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,974,546 C→T T314T (ACC→ACT)  nadR → bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,974,5460CT78.8% 383.4 / 108.2 193T314T (ACC→ACT) nadRbifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/41);  new base T (0/152);  total (0/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.27e-01

Only 100 of 193 total aligned reads displayed.

ATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAA  >  minE/2974512‑2974582
                                  |                                    
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:327528/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:163264/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:156755/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:134143/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:853781/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:237186/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:266612/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:302104/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:313283/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:118955/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:916845/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:387543/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:941830/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:426798/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:964856/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:571988/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:509148/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:526031/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:1010140/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:536902/62‑1 (MQ=255)
aTAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGGTCTGCAAAAAGTACGaa           <  1:992776/62‑1 (MQ=255)
 tAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:198734/61‑1 (MQ=255)
              aTCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:808519/48‑1 (MQ=255)
                 gATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa           <  1:415946/45‑1 (MQ=255)
                           ttGTCACTCCCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1030858/44‑2 (MQ=25)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTTCGAAGGGCGTGag  <  1:855940/44‑2 (MQ=25)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCgagag  <  1:393355/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:610807/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:708993/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:935938/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:657087/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:959127/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:62338/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:615613/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:715486/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:602452/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:575803/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:982750/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:56884/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:987368/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:55795/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:465478/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:871143/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:854445/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:858537/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:848037/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:861130/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:835728/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:864575/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:829363/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:827815/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:713213/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:817910/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:893475/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:802415/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:929160/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:782730/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:773270/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:730480/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1192552/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:284097/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:248886/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:226908/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:206435/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:190261/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:18048/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:12667/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1199672/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1193880/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1193446/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:292625/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1188365/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1163336/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1157842/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1138166/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1120155/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:111058/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1083408/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1019926/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1002930/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:30440/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:533227/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:499159/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:492467/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:485859/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:474855/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:1002783/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:450476/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:446487/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:407679/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:384997/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:381799/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:364142/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:351383/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:350067/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:341032/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:317152/44‑2 (MQ=37)
                           ttGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:308460/44‑2 (MQ=37)
                            tGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:552306/43‑2 (MQ=37)
                            tGTCACTAC‑CAGGCGTTCTGCAAAAAGTACGAAGGGCGTGag  <  1:696820/42‑2 (MQ=25)
                                  |                                    
ATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAA  >  minE/2974512‑2974582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑