Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,048,563	2 bp→GT	coding (1791‑1792/2841 nt)	glnE ←	fused deadenylyltransferase and adenylyltransferase for glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,048,563	0	A	G	100.0%	234.8 / NA	64	L598L (TTG→CTG)	glnE	fused deadenylyltransferase and adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (64/0);  total (64/0)
*	minE	2,048,564	0	C	T	100.0%	217.2 / NA	64	E597E (GAG→GAA)	glnE	fused deadenylyltransferase and adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (64/0);  total (64/0)

ATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCG  >  minE/2048531‑2048592                                 ||                             aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCAt                          >  1:835917/1‑38 (MQ=37) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAg                  >  1:1191633/1‑46 (MQ=37) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGc                >  1:508088/1‑48 (MQ=37) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGcc      >  1:334167/1‑58 (MQ=38) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:617311/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:70678/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:685331/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:680229/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:653770/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:632582/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:630975/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:77111/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:606540/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:581616/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:567/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:536142/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:103988/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:74849/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:503798/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:787542/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:799340/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:802172/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:878748/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:878847/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:931834/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:945216/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:971210/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:971993/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:988483/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:989696/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:995063/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:999029/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:403530/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:10658/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1066611/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1094828/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1101320/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1104222/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1116869/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1131498/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1161467/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1167699/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1185310/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1196446/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:1197160/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:120370/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:138484/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:248625/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:4576/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:401151/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:372308/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:323656/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:281262/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:261498/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:257023/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:251278/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:192313/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:184313/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:177708/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGttg  >  1:171354/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGtt   >  1:149674/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGtt   >  1:663811/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTCATCACGGTAGGCATCCGTCGCCGtt   >  1:812193/1‑60 (MQ=255) aTCTTCCGGCACGCGCAGCAAATACTGGCGCAGTTAATCACGGTAGGCATCCGTCGCCgatg  >  1:771630/1‑59 (MQ=255)                                 ||                             ATCTTCCGGCACGCGCAGCAAATACTGGCGCAACTCATCGCGGTAGGCATCGGTCGCCGTCG  >  minE/2048531‑2048592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑