| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,349,358 | 2 bp→AT | coding (2289‑2290/2787 nt) | polA ← | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,349,358 | 0 | G | A | 100.0% | 14.6 / NA | 6 | L764L (CTG→TTG) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/6); total (0/6) | |||||||||||
| * | minE | 2,349,359 | 0 | A | T | 100.0% | 14.4 / NA | 6 | G763G (GGT→GGA) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/6); total (0/6) | |||||||||||
CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGAC > minE/2349342‑2349403 || cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:1116756/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:1118117/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:647025/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:661213/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:692790/62‑1 (MQ=255)cTCATGCCGTAGATCAATCCGAAGTTGATCGCCTTGGCGCTGCGTCGCTGGTCGCCGCTGAc < 1:794408/62‑1 (MQ=255) || CTCATGCCATAAATCAGACCAAAGTTGATCGCTTTCGCGCTACGGCGTTGCTCGCTGGTGAC > minE/2349342‑2349403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |