| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,445,052 | 2 bp→CT | coding (83‑84/1851 nt) | ilvD ← | dihydroxyacid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,445,052 | 0 | G | C | 100.0% | 16.3 / NA | 6 | T28K (ACC→AAG) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (0/6); total (0/6) | |||||||||||
| * | minE | 2,445,053 | 0 | G | T | 100.0% | 12.1 / NA | 6 | T28K (ACC→AAG) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (0/6); total (0/6) | |||||||||||
GATAATCGGCTTACCGAAATCGGCGTCGGTCATTCCGGTGGCGCGCCACAGCGCACGAGCAC > minE/2445025‑2445086 || gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:236511/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:447497/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:459721/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:466005/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:630416/62‑3 (MQ=255)gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:925371/62‑3 (MQ=255) || GATAATCGGCTTACCGAAATCGGCGTCGGTCATTCCGGTGGCGCGCCACAGCGCACGAGCAC > minE/2445025‑2445086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |