Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,468,230	2 bp→GG	coding (102‑103/864 nt)	atpG →	F1 sector of membrane‑bound ATP synthase, gamma subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,468,230	0	A	G	100.0%	48.8 / NA	15	K34K (AAA→AAG)	atpG	F1 sector of membrane‑bound ATP synthase, gamma subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/0);  total (15/0)
*	minE	2,468,231	0	T	G	100.0%	50.7 / NA	15	S35A (TCG→GCG)	atpG	F1 sector of membrane‑bound ATP synthase, gamma subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/0);  total (15/0)

AAGATCACTAAAGCGATGGAGATGGTCGCCGCTTCCAAAATGCGTAAATCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCA  >  minE/2468183‑2468292                                                ||                                                              aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCa                                                   >  1:974987/1‑61 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCa                                                   >  1:722772/1‑61 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:470438/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:996161/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:861180/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:741164/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:701830/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:471405/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:415620/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:364516/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:1059549/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:222918/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:17261/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:1154262/1‑62 (MQ=255) aaGATCACCAAGGCGATGGAAATGGTCGCCGCATCGAAAATGCGCAAGGCGCAGGATCGCAt                                                  >  1:12587/1‑62 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:605752/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:1056980/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:880484/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:115071/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:841818/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:841734/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:779440/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:757865/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:1173619/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:240583/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:587582/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:568281/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:555939/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:161749/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:1034121/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:37924/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:337927/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCa  <  1:279750/62‑1 (MQ=255)                                                 tCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGGCa  <  1:384517/62‑1 (MQ=255)                                                ||                                                              AAGATCACTAAAGCGATGGAGATGGTCGCCGCTTCCAAAATGCGTAAATCGCAGGATCGCATGGCGGCCAGCCGTCCTTATGCAGAAACCATGCGCAAAGTGATTGGTCA  >  minE/2468183‑2468292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑