Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	25,121	Δ1 bp	coding (151/915 nt)	rihC →	ribonucleoside hydrolase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	25,121	0	C	.	100.0%	197.1 / NA	45	coding (151/915 nt)	rihC	ribonucleoside hydrolase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (45/0);  total (45/0)

AAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAA  >  minE/25100‑25162                      |                                          aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTGTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:72835/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTc             >  1:2183589/1‑51 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGc      >  1:1580618/1‑58 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:288624/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2402638/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2489337/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2506521/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2514626/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2553551/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2567486/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2684949/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2687910/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2884135/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2400696/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2999575/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:346978/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:565793/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:56753/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:67182/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:709341/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:792944/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:797570/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:937521/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1488165/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:102315/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:102678/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1163975/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:126983/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1299004/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1311761/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1316136/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1406574/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1458134/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1004306/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1569052/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1763948/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:1996984/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2044683/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2065999/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2143785/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2307202/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCaa  >  1:2341121/1‑62 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCa   >  1:1732770/1‑61 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCa   >  1:2594242/1‑61 (MQ=255) aaaaCTACCCGCAATGCCCTG‑AACTGCTGCATTTATGGAATGCGGAGATTCCGCTCGCCCaa  >  1:3054271/1‑62 (MQ=255)                      |                                          AAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAA  >  minE/25100‑25162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑