Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,291,760	Δ1 bp	coding (1398/1644 nt)	yeeO ←	predicted multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,291,760	0	A	.	100.0%	301.0 / NA	68	coding (1398/1644 nt)	yeeO	predicted multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/68);  total (0/68)

GGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAA  >  minE/1291748‑1291810             |                                                   ggCTTCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1164115/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTTAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:666138/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCATCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:505615/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCATCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:669480/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGTGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1517374/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGGACCCATGAGGCGGACCAAATAGGCataaa  <  1:1858992/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCAGCCATGAGGCGGACCAAATAGGCataaa  <  1:329969/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:3058298/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:3296274/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:3200119/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:3132452/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:3105774/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:3074470/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:498808/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:296183/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2894084/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2867310/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:28629/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2804242/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2737758/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2675135/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:392174/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:472074/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2593091/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:546581/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:642323/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:753846/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:907499/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:922243/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:922719/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:925641/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:930409/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:931511/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:975408/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:99457/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2559/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1158091/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1164512/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1247324/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1261007/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:140409/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1455831/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1476926/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1524989/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:155921/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1574135/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1629354/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:168530/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1785859/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1812623/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2092291/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1045614/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2509209/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2492322/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2409855/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2323981/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2284852/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:21694/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2158452/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:2031219/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1936938/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1866555/62‑1 (MQ=255) ggCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:182607/62‑1 (MQ=255) ggCATCACAAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:3257737/62‑1 (MQ=255)  gCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1825966/61‑1 (MQ=255)  gCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:1614411/61‑1 (MQ=255)  gCATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCataaa  <  1:552167/61‑1 (MQ=255)   cATCACGAGC‑CCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGTCataaa  <  1:1825514/60‑1 (MQ=255)             |                                                   GGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAA  >  minE/1291748‑1291810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑