Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,307,820	(G)7→6	intergenic (‑225/+42)	yeeF ← / ← yeeY	predicted amino‑acid transporter/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,307,814	0	G	.	100.0%	277.8 / NA	63	intergenic (‑219/+48)	yeeF/yeeY	predicted amino‑acid transporter/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (0/63);  total (0/63)

TTTTTGACAGCGGCGCATTGTGCCTAAATGGGGGGGAAATGACA  >  minE/1307785‑1307828                              |               tttttGACAGCGGCGCTTTGTTCCTAAAT‑GGGGGGAAATGACa  <  1:2345605/43‑1 (MQ=37) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:326306/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2599480/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2693960/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2735335/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2746691/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2914044/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2926220/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3041260/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3075389/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3100387/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3116800/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3141603/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3161174/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3165107/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3179465/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2502968/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:3289781/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:392985/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:393298/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:416521/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:581890/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:655505/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:670904/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:748580/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:762000/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:788982/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:80311/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:891601/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:951337/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:975350/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1925364/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1113016/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1114501/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1173137/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1174558/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1479035/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:149099/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1660532/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1800887/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1844628/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1899782/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1017237/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1955466/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1956545/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2064422/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2476052/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2440885/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2309625/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2295039/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2280277/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2209819/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2094488/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2086775/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2084121/43‑1 (MQ=255) tttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2076290/43‑1 (MQ=255)  ttttGACAGCGGCGCATTGTGCCTAAAT‑GGGTGGAAATGACa  <  1:967702/42‑1 (MQ=38)  ttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:135923/42‑1 (MQ=255)  ttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:377720/42‑1 (MQ=255)  ttttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2004505/42‑1 (MQ=255)   tttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1728026/41‑1 (MQ=255)   tttGACAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:1659981/41‑1 (MQ=255)        cAGCGGCGCATTGTGCCTAAAT‑GGGGGGAAATGACa  <  1:2200782/36‑1 (MQ=38)                              |               TTTTTGACAGCGGCGCATTGTGCCTAAATGGGGGGGAAATGACA  >  minE/1307785‑1307828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑