Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	432,019	+A	coding (385/1407 nt)	ybfM →	predicted outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	432,015	1	.	A	100.0%	103.4 / NA	32	D127E (GAC→GAA)	ybfM	predicted outer membrane porin
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (32/0);  total (32/0)

CCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑AAAAGCGGTATAAGCCTGTA  >  minE/431955‑432035                                                              |                     cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:956064/62‑1 (MQ=255) cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1088980/62‑1 (MQ=255) cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1534815/62‑1 (MQ=255) cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:2271977/62‑1 (MQ=255) cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:2084194/62‑1 (MQ=255) cccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1908761/62‑1 (MQ=255)         aaaTCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1922518/54‑1 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAGGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2615754/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:308518/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2693297/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2802995/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2843418/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2947558/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2960996/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2513436/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:366092/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:391785/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:681878/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:811675/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:84908/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:896063/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:911324/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:96653/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1776051/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1192414/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1199270/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1260665/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1481744/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1612097/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1632970/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1707706/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2652063/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:1853871/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2248656/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2254497/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2350492/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2423465/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTGta  >  1:2566802/1‑60 (MQ=255)                       aaaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAAGCGGTATAAGCCTCta  >  1:2921329/1‑60 (MQ=255)                           gTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑a                     <  1:1599508/36‑1 (MQ=255)                                                              |                     CCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑AAAAGCGGTATAAGCCTGTA  >  minE/431955‑432035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑