| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,126,937 | (A)7→8 | coding (25/45 nt) | pheM ← | phenylalanyl‑tRNA synthetase operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,126,930 | 1 | . | A | 81.5% | 73.0 / 13.5 | 27 | coding (32/45 nt) | pheM | phenylalanyl‑tRNA synthetase operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (0/5); new base A (0/22); total (0/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGT‑A‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTT > minE/1126878‑1126978 | tGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGT‑A‑AAAAaaga < 1:1110287/62‑1 (MQ=255)tGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGT‑A‑AAAAaaga < 1:3075406/62‑1 (MQ=255)tGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGT‑A‑AAAAaaga < 1:1870382/62‑1 (MQ=255)tGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGT‑A‑AAAAaaga < 1:2585200/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:1016843/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:849949/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:708890/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:708833/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:345364/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:340752/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:2941501/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:2716523/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:2655350/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:2638797/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:2278889/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:2209770/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:2056938/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:1761522/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:1455564/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:1445247/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:1337218/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:1127367/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:1109850/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:1043227/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:100574/62‑1 (MQ=255) cAGGTGCTAAAGTAA‑AAAAAAGAAGCGGAAAATAGCAGCATTAATTGCTTGCGTTACCtttt < 1:980482/62‑1 (MQ=255) cAGGTGCTAAAGT‑AGAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCtttt < 1:2875011/62‑1 (MQ=255) | TGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGT‑A‑AAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTT > minE/1126878‑1126978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |