| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,763,594 | 0 | A | T | 71.3% | 153.5 / 194.8 | 223 | noncoding (250/1542 nt) | rrsG | 16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (22/42); new base T (0/159); total (22/201) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.73e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-07 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 223 total aligned reads displayed.
ATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCT > minE/1763540‑1763647 | aTCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAATCTAAt > 1:1758412/1‑62 (MQ=255)aTCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt > 1:169236/1‑62 (MQ=255)aTCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt > 1:2264562/1‑62 (MQ=255)aTCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt > 1:2138932/1‑62 (MQ=255)aTCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt > 1:2588383/1‑62 (MQ=255)aTCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt > 1:2067693/1‑62 (MQ=255) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2717980/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:3070221/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:3048323/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2993487/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2892542/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2877665/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:283417/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2807549/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2780488/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2742409/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:3153097/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2665688/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2534173/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2495878/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2455634/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2436833/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2411402/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2379629/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:529568/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:944424/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:927228/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:819028/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:76996/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:743699/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:69214/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:668866/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:65613/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:622008/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:551404/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:532474/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1071889/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:501406/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:457134/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:445121/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:437074/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:410698/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:350441/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:3275636/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:3240764/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:3208754/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:3191817/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1394063/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1700667/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1678766/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:160451/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1582757/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1576322/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1561483/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1549152/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1460835/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1438395/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1423923/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2322617/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1369315/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1329991/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1306248/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1243503/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1221196/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1129612/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1126245/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1087900/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1044731/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1858648/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1830682/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2289661/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1880201/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2237044/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1966148/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1989683/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2235366/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:200576/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2120565/62‑3 (MQ=2) tcgtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:2053052/62‑3 (MQ=2) gtcgCCTTGGTGAGCCATTACCTCACCAACTAGCTAATCCGACCTAGGCTCATCTGAtag < 1:1293281/60‑3 (MQ=2) gTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGcc > 1:971683/1‑60 (MQ=255) gTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGcc > 1:897080/1‑60 (MQ=255) gTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGcc > 1:2471727/1‑60 (MQ=255) gTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGcc > 1:1163001/1‑60 (MQ=255) gTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGcc > 1:3262912/1‑60 (MQ=255) gTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGcc > 1:1955502/1‑60 (MQ=255) gTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGcc > 1:1948678/1‑60 (MQ=255) ccaccaACAAGCTAATCCCATCTTGGCACATCCGATGGCAAGAGGCCCGAAGGTCCGCctct < 1:3178471/62‑1 (MQ=255) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:2106778/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:2731412/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:2197737/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:587379/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:2754593/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:63432/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:1259061/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:252799/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:1481364/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:368440/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct < 1:2333659/62‑1 (MQ=40) ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGACGCCCGAAGGTCCCCctct < 1:2948763/62‑1 (MQ=255) | ATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCT > minE/1763540‑1763647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |