| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,885,961 | 1 | . | T | 71.2% | 169.7 / 79.7 | 73 | coding (1423/2757 nt) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base . (0/21); new base T (52/0); total (52/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.22e-19 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTCCCGCCGTCATGGTGGCACCGGGCT > minE/1885905‑1885989 | ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:683289/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:2303325/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:392758/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:2423004/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:584571/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:2024273/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:2019899/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:632920/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:680498/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:1728688/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:1691632/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:1677907/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:158570/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:3072109/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:3013049/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:2809146/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:1545971/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:1574968/61‑1 (MQ=255)ttCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:700403/61‑1 (MQ=255) tCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:268040/60‑1 (MQ=255) aCGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTc < 1:878266/54‑1 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:3184801/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:3203371/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:3253051/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:585726/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:3147853/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:3144358/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:3260989/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:3186176/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:370605/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:431848/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:432082/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:519377/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2731758/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:643334/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:681458/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:705285/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:719237/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:759966/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:762389/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:776973/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:867406/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:957947/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1892950/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1102060/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1156188/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1184251/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1526390/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1571199/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1575652/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1644419/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1716555/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1739042/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1799101/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1816614/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:3088438/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1971379/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2435447/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2962331/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2872265/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:1080815/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2700596/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2584576/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2027262/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2392108/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2269738/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2261192/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2219687/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCgggct > 1:2056802/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGGTGGCACCggg > 1:409210/1‑60 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTATTTTCCCGCCGTCATGATGGCACCgggct > 1:2433706/1‑62 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑ttt > 1:678830/1‑36 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑ttt > 1:780549/1‑36 (MQ=255) tATTCCAGGCCTTCCGACAGGCTGAAGCCAGTATTTTCCCGCCGTCATCGTGGCACCg > 1:1357599/1‑58 (MQ=255) tATTCCAGGCCTTCCGACAGGATGATGCCAGTATTTTCCCGCCGTc > 1:1210167/1‑46 (MQ=255) | TTCCTGAACGCGATCAATCGCGCTTATTCCAGGCCTTCCGACAGGCTGATGCCAGTA‑TTTCCCGCCGTCATGGTGGCACCGGGCT > minE/1885905‑1885989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |