| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,968,555 | 0 | A | G | 70.1% | 150.8 / 77.9 | 97 | G25G (GGT→GGC) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base A (29/0); new base G (68/0); total (97/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCA > minE/1968535‑1968609 | cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCAc > 1:2758676/1‑61 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2919577/1‑62 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:1756275/1‑62 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2446932/1‑62 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2717090/1‑62 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:1530709/1‑62 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2986303/1‑62 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:1260875/1‑62 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:416591/1‑62 (MQ=255)cccATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:1103284/1‑62 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCa > 1:1439179/1‑53 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:1029109/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:3220749/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2056424/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2104259/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2223605/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:17365/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:863228/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:1658882/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2696216/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2711967/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:782961/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:2732868/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:710738/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:150974/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:593230/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:3001752/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:3185197/1‑55 (MQ=255) gggCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACg > 1:1333583/1‑55 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGCCGGCGTCCATCGCCAGCGCGCGGATCGCGTTgg > 1:1029384/1‑60 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAgcgcg > 1:3038177/1‑46 (MQ=12) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGcg > 1:615245/1‑55 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGtt > 1:1825290/1‑58 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTgg > 1:1918869/1‑60 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTgg > 1:1739595/1‑60 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTg > 1:108048/1‑59 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:3222683/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:373394/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1251411/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1350951/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:3083850/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:139859/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1474133/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2985829/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2947013/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1476490/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:176212/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2862747/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:3225109/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:3274366/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:3297558/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2864674/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:38819/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:408016/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1210927/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:528930/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:672498/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1149756/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:803236/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1068174/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:879301/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2297942/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1785896/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1802458/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1981556/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2026678/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2054421/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1752677/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2062600/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2073810/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2182052/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2221649/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2272558/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2281545/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2809957/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:168742/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2485056/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2541747/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1529744/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1536074/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1541856/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:1588970/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2634161/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2622530/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2586352/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc > 1:2538670/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:1749030/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:506287/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:2608893/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:547330/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:1168381/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:3090476/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:2964469/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:154776/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:3104548/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:828421/1‑61 (MQ=255) ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc > 1:318310/1‑61 (MQ=255) | CCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCA > minE/1968535‑1968609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |