Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,968,6030AG65.3% 115.0 / 101.1 101N9N (AAT→AAC) tktAtransketolase 1, thiamin‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/35);  new base G (66/0);  total (66/35)
Fisher's exact test for biased strand distribution p-value = 5.97e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 101 total aligned reads displayed.

CCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAC  >  minE/1968548‑1968619
                                                       |                
ccGGGTGGCCCGAGTTGGCTTTCTGGCCGGCGTCCATCGCCAGCGCGCGGATCGCGTTgg              >  1:1029384/1‑60 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGtt                >  1:1825290/1‑58 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTgg              >  1:1918869/1‑60 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTgg              >  1:1739595/1‑60 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTg               >  1:108048/1‑59 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2634161/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2062600/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2622530/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2586352/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2541747/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2538670/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2485056/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2297942/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2281545/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2272558/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2221649/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2182052/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2073810/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1068174/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:3274366/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:879301/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:803236/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:672498/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:528930/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:408016/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:38819/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:373394/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:3297558/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2809957/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:3225109/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:3222683/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:3083850/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2985829/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2947013/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2864674/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2862747/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2026678/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1149756/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1210927/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1251411/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1350951/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:139859/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1474133/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1476490/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1529744/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1536074/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1541856/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1588970/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:168742/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1752677/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:176212/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1981556/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1785896/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:1802458/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGcc            >  1:2054421/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:318310/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:3090476/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:2964469/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:547330/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:1168381/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:3104548/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:506287/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:828421/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:154776/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:2608893/1‑61 (MQ=255)
ccGGGTGGCCCGAGTTGGCTTTCTGGACGGCGTCCATCGCCAGCGCGCGGATCGCGTTGGc             >  1:1749030/1‑61 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCGATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1524290/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:679939/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:499916/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1993054/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2077824/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2089869/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1189176/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:540077/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:3147649/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:729452/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:822330/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:833235/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:835637/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1128302/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:895290/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2898644/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:251129/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2542481/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2614055/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1576187/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2703368/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2772767/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2774731/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1805533/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1530023/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2124960/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2908918/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:3073724/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2181786/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2339050/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:3211237/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:2160197/62‑1 (MQ=255)
          ggATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1410038/62‑1 (MQ=255)
           gATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAc  <  1:1329649/61‑1 (MQ=255)
                                                       |                
CCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAC  >  minE/1968548‑1968619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑