Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,133,882	0	G	T	70.6%	11.7 / 13.5	17	intergenic (‑108/‑179)	ydiY/pfkB	conserved hypothetical protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base T (12/0);  total (17/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTT  >  minE/1133855‑1133915                            |                                  gAAAGGTAACGAATTATAAAAAGGCGCTAATAACt                            >  1:1936863/1‑35 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACt                            >  1:2026435/1‑35 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCa       >  1:642489/1‑56 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:1088904/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:1685058/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:1963644/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:2037221/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:2224102/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:2675275/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:314226/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:407877/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCTAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:460221/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:709351/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:693800/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:1739403/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:1660943/1‑61 (MQ=255) gAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt  >  1:1509983/1‑61 (MQ=255)                            |                                  GAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTT  >  minE/1133855‑1133915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑