Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,170,800	0	G	A	65.3%	51.8 / 46.2	49	A247T (GCT→ACT)	gdhA	glutamate dehydrogenase, NADP‑specific
Reads supporting (aligned to +/- strand):  ref base G (17/0);  new base A (32/0);  total (49/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAATTT  >  minE/1170762‑1170823                                       |                        gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTTCACTATCGAAAAAGCGATGGAAttt  >  1:2210170/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:2001798/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:3004897/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:3194260/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:263931/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:2631994/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:2406564/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:3269309/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:339459/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:2006817/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:1861835/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:34477/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:537641/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:938966/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:1166187/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:691945/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:1524845/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAAttt  >  1:462650/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAg             >  1:2381855/1‑51 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:448578/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:482081/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:348801/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:3249435/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:3243147/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:3218768/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:260951/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:444156/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:746478/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:3146809/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:384811/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1818463/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1072884/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1114928/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1231568/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1249936/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:150642/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1544296/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1679954/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1715752/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:1768135/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:3035116/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:184949/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:2066869/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:2151008/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:2432106/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:2436379/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:2932781/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGATGGAAttt  >  1:2971313/1‑62 (MQ=255) gcgTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACACTATCGAAAAAGCGAGGGAAt    >  1:1006301/1‑60 (MQ=255)                                       |                        GCGTTTCCGTTTCTGGCTCCGGCAACGTCGCCCAGTACGCTATCGAAAAAGCGATGGAATTT  >  minE/1170762‑1170823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑