| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 354,373 | C→G | L230V (CTG→GTG) | cysS → | cysteinyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 354,373 | 0 | C | G | 87.2% | 257.3 / 24.9 | 86 | L230V (CTG→GTG) | cysS | cysteinyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (3/8); new base G (0/75); total (3/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
CTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACC > minE/354324‑354414 | cTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGc > 1:1235105/1‑62 (MQ=255)cTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGc > 1:1481191/1‑62 (MQ=255)cTGCAAGCAGCTGGGGAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGc > 1:2632870/1‑62 (MQ=255) gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac < 1:2701187/62‑1 (MQ=255) gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac < 1:2161134/62‑1 (MQ=255) gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac < 1:1845435/62‑1 (MQ=255) gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac < 1:429608/62‑1 (MQ=255) gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac < 1:2493552/62‑1 (MQ=255) gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac < 1:3180863/62‑1 (MQ=255) gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac < 1:2238592/62‑1 (MQ=255) gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac < 1:16134/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:322196/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:369813/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:3204915/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:3204753/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:3174073/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:3172318/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:3143416/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2992110/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2953549/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2893534/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2891209/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2886417/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2866311/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:286223/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2809153/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:631336/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1106721/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:92452/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:840642/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:793084/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:77300/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:730790/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:700331/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:649263/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:634012/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:328596/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:59597/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:591959/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:527010/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:49114/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:484843/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:434459/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:38536/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:970431/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:349290/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1406364/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:275811/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:188122/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1877689/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1813723/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:17752/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1703069/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1664066/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1541086/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1431341/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1881588/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1338150/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1321822/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1316800/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1297825/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1257363/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1250466/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:124738/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1197791/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1130849/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2008143/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2742482/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2687201/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2684208/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:267303/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2649123/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2348458/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2306346/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2304759/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2242556/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2234494/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2086327/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1972700/62‑1 (MQ=255) tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATAGCGCAGTCCAcc < 1:280073/62‑1 (MQ=255) ccACGGCGGCTGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2184393/61‑1 (MQ=255) ccACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2116388/61‑1 (MQ=255) gcggcggTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:1786064/56‑1 (MQ=255) ggcggTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2767677/54‑1 (MQ=255) gcggTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:2736584/53‑1 (MQ=255) tCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc < 1:3050808/48‑1 (MQ=255) | CTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACC > minE/354324‑354414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |