Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	395,054	G→A	V135V (GTG→GTA)	ahpF →	alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	395,054	0	G	A	100.0%	105.4 / NA	33	V135V (GTG→GTA)	ahpF	alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/0);  total (33/0)

CTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGC  >  minE/395030‑395093                         |                                        ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:2130865/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:807017/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:756111/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:66173/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:6468/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:3286014/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:3241117/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:3033834/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:3025397/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:287441/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:2794055/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:2652081/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:25088/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:2371180/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:234429/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:2218416/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1470097/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1096592/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1101404/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1164804/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1250553/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1365553/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1448200/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1453705/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1030865/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1624049/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1783554/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:17920/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1827934/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1878153/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:1881749/1‑61 (MQ=255) ctctTGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcg     >  1:206908/1‑61 (MQ=255)    ttGCCACAACTGCCCGGACGTAGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCgcgc  >  1:411062/1‑61 (MQ=255)                         |                                        CTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGC  >  minE/395030‑395093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑