Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 462,043 G→A R10H (CGC→CAC)  sdhD → succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE462,0430GA100.0% 499.0 / NA 143R10H (CGC→CAC) sdhDsuccinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (72/71);  total (72/71)

Only 100 of 144 total aligned reads displayed.

CACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTC  >  minE/461993‑462098
                                                  |                                                       
cACTTCTCGCAGGAGTCCTCGTATGTTAAGCAACGCCTCCGCATTAGGACACAATGGCGTa                                               >  1:2160825/1‑61 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGAcaca                                                       >  1:210533/1‑53 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGt                                                >  1:1148835/1‑60 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGt                                                >  1:2463693/1‑60 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTa                                               >  1:726797/1‑61 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTa                                               >  1:654820/1‑61 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTa                                               >  1:644900/1‑61 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTa                                               >  1:15047/1‑61 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTa                                               >  1:3282901/1‑61 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2787882/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:3063121/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2713085/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2636968/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2615302/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2535153/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2403069/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2386501/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2282841/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2242364/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2162526/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2876372/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2965058/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2998513/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1028875/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:3163632/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:3205208/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:343064/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:457927/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:586808/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:605429/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:609747/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:623115/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:713535/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:876947/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2062475/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2083589/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1622493/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1679071/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1711727/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1266091/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1202048/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1802462/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1195211/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1818165/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2058597/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1042818/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1003612/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:190133/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:1943862/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCACCGCATTAGGACACAATGGCGTAc                                              >  1:454057/1‑62 (MQ=255)
cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGACTCCGCATTAGGACACAATGGCGTAc                                              >  1:451415/1‑62 (MQ=255)
cACTTATCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACACAATGGCGTAc                                              >  1:2367110/1‑62 (MQ=255)
                              cAACGCCTCCGCATTAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTa                <  1:1333698/62‑1 (MQ=255)
                              cAACGCCTCCGCATTAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTa                <  1:623670/62‑1 (MQ=255)
                                            tAGGACACAATTGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2360435/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:962125/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1295634/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:420595/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:419027/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:3025352/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:357148/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:3260841/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1479546/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:447054/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:45118/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:565330/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:919078/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1187217/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1087818/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:625128/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1151436/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1121298/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1485368/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2111595/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2184348/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1843584/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2317757/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2326320/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:236240/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1826427/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2396090/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1813531/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2484422/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2485379/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2562619/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1727658/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2676731/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2685966/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1684530/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2853451/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1628139/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:2929939/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:1525164/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:302487/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:151686/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:3121572/62‑1 (MQ=255)
                                            tAGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:3201811/62‑1 (MQ=255)
                                            tAGGACACAATCGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:131728/62‑1 (MQ=255)
                                            tAGCACACAATCGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:3273197/62‑1 (MQ=255)
                                             aGGACACAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGctc  <  1:968266/61‑1 (MQ=255)
                                                  |                                                       
CACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTC  >  minE/461993‑462098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑